With the increase in pesticide wastewater of compounds that are difficult to biodegrade conventional biochemical treatment methods cannot completely remove contaminants from wastewater.

Conclusions: to make sure that assay result of routine biochemistry items is more accurate, we advise to adopt multi-points calibration model in place of traditional single-point calibration model.

Objective To evaluate the influence of five types of evacuated and promoting coagulating tubes on the clinical routine biochemistry test.