Objective To explore Insulin like growth factor 1 receptor(IGF 1R)gene quantitative expression in human pancreatic cancer cells(PC 3) and the relationship of IGF 1R with apoptosis?tumorigenicity.
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方法原代分离人胰岛素瘤细胞,经多次筛选培养进行纯化。
MethodsPrimary human insulinoma cells were isolated by using collagenase type p, and then purified by repeatedly selective cell culture.