More free sulfhydryls and higher sample concentration mean that one should consider using higher concentrations of reducing agent.
2
在确定还原剂和样品比例时,需要考虑样品上自由巯基的数目和样品浓度。
When deciding on the appropriate concentration of reducing agent for the sample, consider the number of free sulfhydryls in the sample as well as sample concentration.
3
有报道指出还原剂作为没有自由巯基的蛋白的添加剂的情况下长出了晶体的成功案例。
There have been reports where reducing agents have been used as successful crystallization additives where there were no free sulfhydryls in the sample.