METHODS: Colorimetric analysis, was performed by the anthrone reagent.
2
用蒽酮比色法和酚硫酸法测定产品糖含量,考马斯亮蓝法测定蛋白质含量。
The reactions of saccharide with Anthron and phenol_sulphate acid are used to analyze saccharide content. The content of protein in the products is determined by Coomassie Brilliant Blue method.
METHODS: The contents of total alkaloids, diester diterpenoid alkaloids and polysaccharides were measured by acid dye colorimetry, HPLC and sulphuric acid-anthrone colorimetric method respectively.