Study the cell-wall degrading enzymes (CWDEs) and cutinase by Botrytis cinerea and their pathogenicity to plant could make us best understanding about pathogenic mechanisms of plant pathogens.
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方法：采用分离酶和胰蛋白酶联合消化法分离小型香猪角质形成细胞，角质形成细胞培养基培养。
METHODS: Keratinocytes of mini fragrant pigs were separated by dispase and trypsin combined digestive method, and then cultured with keratinocyte culture medium.
Malassezia can breakdown lipid like esterase, and result in morphological changes of malpighian cell and cell apoptosis, these are all their main etiological factors.
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