The extraction technology and physical-chemical properties of honeybee pupae SOD were studied in this research.

Extraction and purity of superoxide dismutase (SOD) by different method are demonstrated, and a good method with high activity and purification multiple has been found.

In order to improve the value of the multiple use, the processes of extracting superoxide dismutase (SOD) from maize (without) affecting ethanol fermentation were studied.