Results During long term cultivation, the population doubling time of the cells became shorter and the percentage of aneuploidy chromosomes and colonies grown in soft AGAR increased.

结果卵圆细胞在培养过程中倍增时间逐渐缩短，非整倍体染色体数目增加，在软琼脂中生长的克隆增多。

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Results Population doubling time of corneal stromal cells prolonged significantly since passage 7. MTT assay also showed cell viability of passage 7 decreased to 60% of passgage 1 (P< 0. 01 ).

细胞群体倍增时间由第7代起显著延长（P<0.01）； MTT比色法的结果亦显示，第7代细胞的增殖能力开始大幅下降（P<0.01）。

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