...子-α；增殖；碱性磷酸酶 [gap=1315]Key words:dental follicle cell; Tumor necrosis factor-α; proliferation; ALP activity ...
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Then cell proliferation was observed by microtitration (MTT), ALP activity was detected through PNPP method, and the number of mineralized node were counted after stained by von Kossa.
MTT法测定OB增殖情况,PNPP法测定细胞内碱性磷酸酶(ALP)活性,改良von Kossa染色计数矿化结节。
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ALP activity and calcium content.
碱性磷酸酶活性及钙含量。
Results: the active components extracted by alcohol strengthened the proliferation ability and ALP activity of cultured human periodontal ligament cells.
结果：补肾中药醇提活性成分对人牙周膜细胞增殖能力和ALP活性有增强作用。
Conclusions: SD rat Cementoblast-like has the unique biological characteristic expressing mineral-associated proteins such as OCN and lacking ALP activity.
结论：体外培养的类成牙骨质细胞表达矿化相关蛋白，但几乎没有碱性磷酸酶活性；
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