The positive recombinant plasmid was identified by PCR, enzyme digestion. The nucleotide sequence of CSP3 'ending gene was determined by the dideoxy chain termination method.
阳性重组质粒经pcr、酶切鉴定，用双脱氧链末端终止法进行序列测定。
The result of the nucleotide sequence determined by dideoxy chain-termination method indicated that 5 '-terminal nucleotide sequence of the amplified NS1 fragment was not changed.
用双脱氧链末端终止法测序结果表明，NS 1基因扩增片段5端的部分碱基序列并未发生改变。
The result of the nucleotide sequence determined by dideoxy chain-termination method indicated that 5'-terminal nucleotide sequence of the amplified NS1 fragment was not …
用双脱氧链末端终止法测序结果表明，NS1基因扩增片段5’端的部分碱基序列并未发生改变。
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