中国最大的综合性文献数据库 -维普资讯 关键词: 基因破坏 酿酒酵母 PCR[gap=1319]Key words: Gene Disruption; Saccharomyces cerevisiae; PCR
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白念珠菌菌落PCR方法的建立和应用_中国家具网 关键词:白念珠菌;菌落PCR;基因敲除;基因型验证 [gap=715]Key words: Candida albicans; colony PCR; gene disruption; genotype confirmation
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酵母基因中断技术(gene disruption)是基于酿酒酵母同源重组这一原理,通过一定的手段使目标基因完全或部分缺失,并在此基础上研究缺失突变体表型变化或生理代谢变化。
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gene-disruption 基因敲除
gene disruption technique 基因中断技术
gene disruption and replacement 基因破坏和基因替换
gene double disruption 基因双敲除
disruption gene 破裂基因
In this study, PCR-mediated gene replacement technique was applied in theconstruction nsdA gene disruption strain.
本研究采用了PCR介导的基因置换技术构建nsdA基因中断菌株。
参考来源 - nsdA基因中断及orfX基因整合表达对阿维链霉菌次级代谢的影响·2,447,543篇论文数据,部分数据来源于NoteExpress
The results show that the stable leu2 mutants have been constructed successfully by one-step gene disruption.
结果表明,用一步基因中断法成功地建立了稳定的LEU2基因突变体。
Most importantly, such a precise gene disruption in the nervous system will allow us to study specific roles of candidate genes in neurodegenerative diseases that afflict the aging.
更为重要的可能在于,在神经系统中有目的干扰某个基因的表达,对影响寿命的有关神经退行性疾病的研究将会有所助益。
It is marked by a mutation in a gene known as RPE65 which results in a missing enzyme that leads to degradation of the retina and the disruption of vision processing in the brain.
这种病的特点是,一段名为RPE65的基因发生变异,导致缺失,进而引起视网膜退化,大脑视觉处理中断。
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