医学论文范文:人前脑啡肽原绿色荧光蛋白真核表达载体的构建 关键词】 绿色荧光载体;真核表达;人前脑啡肽原 [gap=990]Key words:green fluorescent vector;eukaryotic expression; PENK
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green fluorescent protein vector 绿色荧光蛋白表达载体
green fluorescent protein fusion vector 绿色荧光蛋白融合载体
green fluorescent protein expression vector 绿色荧光蛋白表达载体
Objective To construct the retroviral(RV) vector with report gene enhanced green fluorescent protein(EGFP) and to explore the gene transfection efficiency of RV on SK-N-SH neuroblastoma cells.
目的构建携带报告基因增强型绿色荧光蛋白（EGFP）的反转录病毒载体，并且探讨病毒载体对SK-N-SH神经母细胞瘤细胞株的感染效率。
The vector is in ring shape , comprising a procaryon replication origin, two eucaryon replication origins and selective marker genes, and two green fluorescent protein gene expression cassettes;
该载 体是一种环状载体，包含原核复制起点、真核复制起点、筛选标记基因和绿色荧光蛋 白基因表达盒；
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