Objective: to construct cloning and prokaryotic expression vector of vascular basement membrane-derived multifunctional peptide and to analyze its space conformation.
目的:构建血管基膜衍生多功能肽克隆和原核表达载体,并对血管基膜衍生多功能肽氨基酸序列进行空间结构分析预测。
We isolated and purified EBP effectively, and obtained EBP with biological activity for the first time with gene cloning method and prokaryotic expression system.
通过基因克隆等方法,选择原核表达系统,并对目的蛋白进行分离纯化,初步获得有活性的人内毒素结合肽ebp。
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