The results show that there is hypochromic effect on the subtract UV spectrum and quenching of fluorescence intensity of riboflavin with increase of concentration of DNA.
研究结果表明，随着DNA浓度增加，核黄素产生紫外光谱减色效应及荧光猝灭效应。
The common used method of getting the absorption spectrum is to subtract the background spectrum from the transmission spectrum of the sample.
得到待测样品吸收光谱的通常方法是，将样品的透射光谱和不含样品的背景光谱进行差减计算。
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