If surface-active substances are used for sample preparation, their absence of toxicity for micro-organisms and their compatibility with inactivators used must be demonstrated.

如果在供试品的制备过程中使用了表面活性剂， 必须证明其不会对微生物产生毒性并且可与 灭活剂兼容。

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Whether the presence of acid conditions or surface active digestive substances in the guts of such marine organisms can desorb and release contaminants in significant quantities to cause such effects, or whether such a response is to their physical presence, remains to be answered.

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