A method named selective amplification DNA fragments (SADF) using single restrictive enzyme was established and optimized in barley.
建立和优化了大麦dna单限制性酶切选择性扩增多态性技术体系(SADF)。
It relies upon the selective deamination of unmethylated cytosine to uracil after treatment with sodium bisulphite, usually followed by PCR amplification of the chosen target region.
它依赖于用 亚硫酸氢钠处理后，非甲基化的 胞嘧啶选择性的去氨基为 尿嘧啶，通常接着所选的靶向区域的PCR扩增。
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